Indoor growingJ. Advances in Biology & Biotechnology · Aug 2026
Red–blue LED spectra and photoperiod shorten crop duration and advance flowering in hydroponic arugula
Light is the biggest lever and the biggest running cost in an indoor farm. We grew two arugula cultivars under four red and blue LED recipes and an outdoor control to find which settings grow the most and finish the soonest.
Obul Reddy Sravani, A. Manohar Rao, Ch. Shanmukhi, Mithun Chakraborty
17 daysshorter growing cycle under the best LED recipe than outdoors
27 daysearlier flowering for cultivar ES2 under 2 blue : 1 red, 18 hours
2R:1Bat 16 hours gave the most leaf in both cultivars
Research question
How do red:blue ratio and photoperiod together shape biomass and flowering in Eruca sativa?
Red and blue LEDs drive growth, leaf size and flowering, but the best mix depends on the crop and the cultivar. For arugula there was little data on how red-heavy and blue-heavy light, run for 16 or 18 hours a day, changes biomass and timing.
Methods
Factorial CRD: two cultivars × five light regimes, three replications, NFT hydroponics
Three stages: grow, light, measure.
GrowTwo cultivars, ES1 and ES2, in a hydroponic NFT system at our Hyderabad facility.
LightFour LED recipes: 2 red : 1 blue or 2 blue : 1 red, for 16 or 18 hours, plus an outdoor control.
MeasureRoot and leaf biomass, days to the end of growth and days to first flower, 3 replicates.
Results
Flowering advanced by up to 27 days under 2B:1R at 18 h (cultivar ES2)
Every LED recipe beat the outdoor control on biomass, growing time and flowering time.
Figure 1
Days to first flower
Fewer is faster. Mean of three replicates.
ES1ES2
Outdoor control
96.2
95.7
2 red : 1 blue, 18 h
85.9
92.6
2 red : 1 blue, 16 h
87.2
79.5
2 blue : 1 red, 18 h
86.3
68.7
2 blue : 1 red, 16 h
79.7
76.0
0255075100
Data: Table 4 of the paper.
Results
Mean crop duration fell from 91.3 days to 74.4 days under 2B:1R at 18 h
Blue-heavy light at 16 hours gave the most root mass; red-heavy light at 16 hours gave the most leaf.
Figure 2
Days to complete growth
Mean of both cultivars.
Outdoor control
91.3
2 red : 1 blue, 18 h
74.6
2 red : 1 blue, 16 h
75.1
2 blue : 1 red, 18 h
74.4
2 blue : 1 red, 16 h
76.3
0255075100
Data: Table 3 of the paper.
Implications
Optimal light regimes are cultivar-specific
The best recipe differed by cultivar, so light settings should be tuned to the variety, not just the crop. A recipe that finishes a crop 17 days sooner frees shelf space and power in every indoor farm cycle.
17 daysshorter growing cycle under the best LED recipe than outdoors
27 daysearlier flowering for cultivar ES2 under 2 blue : 1 red, 18 hours
2R:1Bat 16 hours gave the most leaf in both cultivars
Research question
GrowTwo cultivars, ES1 and ES2, in a hydroponic NFT system at our Hyderabad facility.
LightFour LED recipes: 2 red : 1 blue or 2 blue : 1 red, for 16 or 18 hours, plus an outdoor control.
MeasureRoot and leaf biomass, days to the end of growth and days to first flower, 3 replicates.
Figure 1
Days to first flower
Fewer is faster. Mean of three replicates.
ES1ES2
Outdoor control
96.2
95.7
2 red : 1 blue, 18 h
85.9
92.6
2 red : 1 blue, 16 h
87.2
79.5
2 blue : 1 red, 18 h
86.3
68.7
2 blue : 1 red, 16 h
79.7
76.0
0255075100
Data: Table 4 of the paper.
Figure 2
Days to complete growth
Mean of both cultivars.
Outdoor control
91.3
2 red : 1 blue, 18 h
74.6
2 red : 1 blue, 16 h
75.1
2 blue : 1 red, 18 h
74.4
2 blue : 1 red, 16 h
76.3
0255075100
Data: Table 3 of the paper.
17 daysshorter growing cycle under the best LED recipe than outdoors
27 daysearlier flowering for cultivar ES2 under 2 blue : 1 red, 18 hours
2R:1Bat 16 hours gave the most leaf in both cultivars
The paper
Effect of Light Spectra and Photoperiod on Flowering and Biomass Accumulation in Arugula (Eruca sativa L.)
Read the abstract
Optimising light quality and photoperiod is a critical approach to improving crop growth, yield, and flowering in controlled-environment agriculture, where artificial lighting serves as the primary energy source for plant development. The present study evaluated the influence of different LED light spectra and photoperiods on biomass accumulation and flowering behaviour in arugula (Eruca sativa L.) cultivated under indoor farming conditions. Two cultivars, ES1 and ES2, were grown in a hydroponic production system and exposed to five lighting treatments comprising different red-to-blue and blue-to-red light ratios under 16-h and 18-h photoperiods, including a control treatment. The experiment was conducted using a factorial completely randomised design (FCRD) with three replications. Growth and reproductive responses were assessed by recording fresh leaf biomass, fresh root biomass, crop growth duration, and days to flowering. Statistical analysis revealed significant differences among light treatments, cultivars, and their interactions for all measured parameters. The 2B:1R light spectrum with a 16-h photoperiod produced the highest fresh root biomass, while the 2R:1B spectrum with a 16-h photoperiod produced the highest leaf biomass. Modified red and blue light combinations also reduced crop growth duration and promoted earlier flowering compared with the control, although the most effective treatment varied between cultivars. Overall, ES2 showed greater biomass accumulation and earlier flowering than ES1 under most optimised lighting conditions. These findings indicate that appropriate manipulation of LED light spectra and photoperiod can improve vegetative growth and reproductive development in arugula and provide useful guidance for controlled indoor production.
Cite as
Sravani, O. R., Rao, A. M., Shanmukhi, C., & Chakraborty, M. (2026). Effect of light spectra and photoperiod on flowering and biomass accumulation in arugula (Eruca sativa L.). Journal of Advances in Biology & Biotechnology, 29(9), 69–76. https://doi.org/10.9734/jabb/2026/v29i94284
Published in
Journal of Advances in Biology & Biotechnology, vol. 29, no. 9, pp. 69 to 76. Published 11 August 2026